Stop Solution for TMB Substrate from BioLegend

Product Specs
ItemStop Solution for TMB Substrate
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423001
Quantity60 mL
ApplicationsELISA
TypeSOLUTION

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.solution (421101, BioLegend) was added per well. Upon sufficient color development, 100 μL stop solution (423001, BioLegend) was added. Absorption was read at 450 nm. 4.6. Animals Adult female inbred Lewis rats

(2) Crosstalk between ILC2s and Th2 cells varies among mouse modelsCell reportsFebruary 28, 2023Gurram RK, Wei D, Yu Q, Butcher MJ, Chen X, Cui K, Hu G, Zheng M, Zhu X, Oh J, et al.with the TMB substrate solution (Cat# 421101; Biolegend). The stop solution for TMB substrate (Cat# 423001; Biolegend) was used to terminate the reaction. Final absorbance was measured at 450 nm. The concentration

(3) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.(x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop Solution,Biolegend,Cat#423001 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NativePAGE™ Sample Buffer (4X

(4) Inflammation and neutrophil extracellular traps in cerebral cavernous malformationCellular and Molecular Life SciencesApril 1, 2022Anthony C. Y. Yau et al.then incubated with 3,3',5,5'-tetramethylbenzidine (T0440; Sigma-Aldrich) in the dark. Stop solution (423001; Biolegend) was added to terminate the signal development. The absorbance was determined at 450 nm,

(5) A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and MechanismsCancers (Basel)February 3, 2022Anissa Nofita Sari et al.(BioLegend Inc., BioLegend Way, San Diego, CA, USA) for 30 min, followed by the addition of 100 µL Stop Solution (423001) (BioLegend Inc., BioLegend Way, San Diego, CA, USA). Optical density was measured at 450 nm using an

(6) In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapyScience AdvancesJanuary 21, 2022Jeong Pil Han et al.substrate (BioLegend, 421101). After stopping the HRP/TMB reaction using stop solution (BioLegend, 423001), the plate was subjected to absorbance measurement at 450 nm. Commercial mouse anti-Cas9 antibody (Abcam,

(7) Evaluating TNF-α and Interleukin-2 (IL-2) Levels in African American Primary Open-Angle Glaucoma PatientsGenes (Basel)December 25, 2021Teja Alapati, Kyra M. Sagal, Harini V. Gudiseva, Maxwell Pistilli, Mark Pyfer, Venkata Ramana Murthy Chavali, Joan M. O’Brienincubated in the dark for 20 min. The reaction was stopped by adding 100 µL of stop solution (BioLegend No. 423001, Biolegend, San Diego, CA, USA) to each well. Within 15 min, plate absorbance was measured at 450 nm

(8) Landscape and selection of vaccine epitopes in SARS-CoV-2Genome MedicineDecember 1, 2021Christof C. Smith et al.(Thermo Fisher Scientific cat. #34028) was added, development was stopped with TMB Stop solution (BioLegend cat. #423001), and plates were read at 450 nm. ELISpot Mouse vaccination All mouse work was performed according

(9) A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case-control studyPLoS medicineDecember 1, 2020Debernardi S, O'Brien H, Algahmdi AS, Malats N, Stewart GD, Plješa-Ercegovac M, Costello E, Greenhalf W, Saad A, Roberts R, et al.the manufacturer’s instructions. TMB Substrate Set and Stop Solution from BioLegend (Cat# 421101 and 423001) were used for REG1B quantification. Plasma samples were diluted 1:5 and 1:10 for all 4 ELISAs. Optical

(10) Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol DerivativesInternational journal of molecular sciencesJuly 30, 2020Malik V, Garg S, Afzal S, Dhanjal JK, Yun CO, Kaul SC, Sundar D, Wadhwa R.CA, USA, 421101) for 30 min. One hundred microliters of Stop Solution (BioLegend, San Diego, CA, USA, 423001) was added, and optical density was measured at 450 nm using a spectrophotometer. Differences in VEGF

(11) Interrogating the recognition landscape of a conserved HIV-specific TCR reveals distinct bacterial peptide cross-reactivityeLifeJuly 27, 2020Mendoza JL, Fischer S, Gee MH, Lam LH, Brackenridge S, Powrie FM, Birnbaum M, McMichael AJ, Garcia KC, Gillespie GM.(2) for UV-peptide exchange ELISA (100 μL/well) "Chemical compound, drug",Stop Solution,Biolegend,423001,Assay development stop step (3) for UV-peptide exchange ELISA (100 μL/well) "Software, algorithm",FLUOstar,BMG

(12) Atypical memory B-cells are associated with Plasmodium falciparum anemia through anti-phosphatidylserine antibodieseLifeNovember 12, 2019Rivera-Correa J, Mackroth MS, Jacobs T, Schulze Zur Wiesch J, Rolling T, Rodriguez A.PRISM,, Other,Phosphatidylserine,Sigma-Aldrich,P7769,20 μg/ml Other,Calf Thymus DNA,Sigma-Aldrich,D4522,10 μg/ml Other,Stop buffer,Biolegend,423001, Other,Annexin V,Biolegend,640902,0.5 µM Other,X- VIVO 15 media,Lonza,04-418Q, Other,Human AB healthy

(13) Express ELISA for detection of mortalinBioTechniquesOctober 1, 2019Garg S, Wu C, Ohmiya Y, Kaul SC, Wadhwa R.substrate (421101, BioLegend; CA, USA) for 45 min for color development. 100 μl/well stop solution (423001, BioLegend) was added onto the substrate and mixed, and optical density was measured at 450 nm using

(14) Removal of N-Linked Glycosylation Enhances PD-L1 Detection and Predicts Anti-PD-1/PD-L1 Therapeutic EfficacyCancer cellAugust 12, 2019Lee HH, Wang YN, Xia W, Chen CH, Rau KM, Ye L, Wei Y, Chou CK, Wang SC, Yan M, et al.TMB High Sensitivity Substrate Solution,BioLegend,Cat#421501 STOP Solution,Cell Signaling Technology,Cat#7002 Stop Solution for TMB Substrate,BioLegend,Cat#423001 BSA blocking solution for ELISA,Santa Cruz Biotechnology,Cat#293965 Animal-Free Blocking Solution,Cell Signaling Technology

(15) Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are DamagingImmunitySeptember 18, 2018Stewart I, Radtke D, Phillips B, McGowan SJ, Bannard O.,Thermo fisher scientific,Cat# A20173 TMB Substrate Set,Biolegend,Cat# 421101 Stop solution for TMB substrate,Biolegend,Cat# 423001 eBioscience Foxp3 / Transcription Factor Staining Buffer Set,Invitrogen,Cat# 00-5523-00 Fixation/Permeabilization Solution Kit

(16) Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damagePLoS geneticsFebruary 1, 2018Burger K, Gullerova M.

(17) Identification and Functional Characterization of Anti-metastasis and Anti-angiogenic Activities of Triethylene Glycol DerivativesFrontiers in oncologyJanuary 1, 2018Oh E, Garg S, Liu Y, Afzal S, Gao R, Yun CO, Kaul SC, Wadhwa R.with TMB substrate (BioLegend, 421101) for 30 min for color development. Stop solution (BioLegend, 423001) was added onto the substrate and mixed. The optical density was measured at 450 nm using Tecan infinite

(18) Nasal vaccination with poly(β-amino ester)-poly(d,l-lactide-co-glycolide) hybrid nanoparticlesInternational journal of pharmaceuticsAugust 30, 2017Sinani G, Sessevmez M, Koray Gök M, Özgümüş S, Okyar A, Oya Alpar H, Cevher E.

(19) A novel method for assaying human regulatory T cell direct suppression of B cell effector functionJournal of immunological methodsFebruary 1, 2017Weingartner E, Courneya JP, Keegan A, Golding A.(eBiosceince, Catalog #00–4201-56) was added followed by 100 μL of Stop Solution (Biolegend, Catalog #423001). Absorbance at 450 nm was measured on a standard ELISA reader. A standard curve using human IgM (Sigma,

(20) Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neuronsMolecular neurodegenerationOctober 5, 2016Pigoni M, Wanngren J, Kuhn PH, Munro KM, Gunnersen JM, Takeshima H, Feederle R, Voytyuk I, De Strooper B, Levasseur MD, et al.

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Stop Solution for TMB Substrate from BioLegend

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Stop Solution for TMB Substrate from BioLegend

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